In the graphical display mode, the construct can be displayed as linear or circular, and each segment of the construct can be defined and displayed in varying thickness, pattern, shape, direction, and color. Restriction enzyme sites can be found and marked automatically, and site markers can be automatically arranged. Regions of interest (coding regions, introns, exons, promoters, etc.) can be denoted with arrows or lines. Several layers of regions of interest can be displayed. Any segment of DNA between two marked sites can be selected, copied, cut, pasted, and edited. GCK features a magnification tool - which can be set to a specific number of nucleotides/cm or can be set to fit the construct to the window or to the printer page. Zooming in or out presents views of the construct at any level of magnification, allowing viewing, editing, or printing at any level of detail. A scale can be shown if desired. The same contact form may be used to request pricing details (academic, government & commercial).The Gene Construction Kit® (GCK) program has been the preferred plasmid mapping software of leading researchers for more than 20 years. GCK allows easy manipulation of DNA sequences, either graphically or as sequence text - quickly saving users both time and money. GCK eliminates tedious examination of DNA sequence data by automatically identifying open reading frames (ORF’s), keeping track of sticky ends during cutting and pasting of restriction enzyme digestion fragments, assisting with PCR primer design, and enabling comprehensive annotation of DNA sequence features. This DNA analysis software allows multiple files to be opened and displayed simultaneously, allowing DNA sequences to easily be copied and pasted between plasmids and vectors to represent real-world DNA cloning protocols. The Gene Construction Kit software is available for both Windows and Macintosh users, and files can be shared across platforms allowing for easy collaboration. The interface and set of functions in GCK will significantly enhance laboratory productivity and minimize experimental design errors - saving users both time and money.ĭNA can be represented and manipulated in either graphical or sequence/text modes - with any formatting or sequence manipulation changes made in one view, automatically being applied to the selection in the alternate view. Users have complete control over the display in either mode. Segments can be selected in either mode and cut, copied, or pasted. When a selected segment is cut or copied, the ends are defined by the sites used to delimit the segment. Gene Construction Kit automatically monitors sticky ends that can be generated by restriction enzymes. Incompatible ends cannot inadvertently be pasted together. If such an attempt is made, GCK utilizes a unique "ligation dialog" to assist users in adjusting the ends for compatibility. Utilizing the special paste option allows a sequence to be inverted and/or to have the ends filled or trimmed before pasting, thus circumventing the ligation dialog if the manipulations warrant it. A full trial version is available upon application to the developer. The demo version available from the above link has limited functions (printing, saving, and importing/exporting files are not enabled). The interface and set of functions in GCK will significantly enhance laboratory productivity and minimize experimental design errors - saving users both time and money. The Gene Construction Kit software is available for both Windows and Mac users, and files can be shared across platforms allowing for easy collaboration. This DNA analysis software allows multiple files to be opened and displayed simultaneously, allowing DNA sequences to easily be copied and pasted between plasmids and vectors to represent real-world DNA cloning protocols. GCK eliminates tedious examination of DNA sequence data by automatically identifying open reading frames (ORF's), keeping track of sticky ends during cutting and pasting of restriction enzyme digestion fragments, assisting with PCR primer design, and enabling comprehensive annotation of DNA sequence features. GCK allows easy manipulation of DNA sequences, either graphically or as sequence text - quickly saving users both time and money. The Gene Construction Kit program has been the preferred plasmid mapping software of leading researchers for more than 20 years.
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